To identify factors that influence the patient safety climate of a hospital institution. Cross-sectional study carried out with a multidisciplinary team of a private hospital in the Central-West of Brazil in 2019. An instrument covering sociodemographic and work aspects and the Safety Attitudes Questionnaire – Short Form 2006 were used for data collection. The data were analyzed through the Kruskal Wallis, Mann-Whitney, Spearman’s, Nemenyi and Fisher’s tests. 293 healthcare providers participated in the study, with a mean age of 36 years, 72.01% female, and 35.49% with complete high school education. Data revealed an unfavorable patient safety climate (mean=73.53). Among the domains, job satisfaction presented the highest score (mean=83.28), while perception of the management of the unit and the hospital showed greater fragility (mean=67.40). The domain Patient safety training in the last 12 months led to an mean increase of 5.64 in the patient safety climate. A negative perception of the patient safety climate was found, with a significant influence from patient safety training and the intention to leave the job. The evidence confirms the importance of continuous investments in patient safety training and research, as trained professionals have a more critical and wider view of safety processes, a positive factor for constructive and sustainable changes.